We can use standard TEM for general observation of cells, contrasted or not contrasted samples. Correlative microscopy from fluorescent microscope to TEM offers further method to investigate biological samples .
We have the possibility to observe 2D images, but 3D tomography allows to reconstruct the volume. High resolution reconstruction of viruses is another interesting biological application. As the sample is very beam sensitive, it can be prepared and embedded in vitreous ice, and directly transferred in the TEM. This technique is called CRYO Microscopy. Fully automatic recipe can be used also for automatic image acquisitions.
State of the art TEMs will be presented on both standard and high end instruments, automatic acquisitions will be introduced.